Police say that a substance, believed to be powdered paint, was sprayed on an area inside the abbey on Monday (January 13).
Alyson Lee of Park Grove in Derby, and Diane Bligh, of Langham Place in Frome, have been bailed to appear at Westminster Magistrates’ Court charged with criminal damage.
Lee, 66, will appear in court on February 11 and Bligh, 77 will appear on February 12.
Climate protest group Jus Stop Oil said two activists used chalk paint on the grave of Charles Darwin inside the abbey.
The slogan “1.5 is dead” was marked on the 19th-century biologist’s grave at about 9.30am during the protest.
A Westminster Abbey spokesperson said: “The Abbey’s conservators are taking immediate action to clean the memorial and do not anticipate that there will be any permanent damage.”