The heritage site, known as the Cathedral on the Marshes, will host the celebrations on Saturday, April 5, and Sunday, April 6.
The events will include the steaming of the Prince Consort beam engine, special talks, exhibitions, and live music reminiscent of the Victorian era.
A pop-up bar by Bexley Brewery will also add to the festive atmosphere.
Crossness Engines, built to manage London’s sewage, is a masterpiece of Victorian engineering with four large steam-driven beam engine pumps housed in an ornate engine house.
Over the years, it has seen the addition of an exhibition space, café, and event areas.
Councillor Cafer Munur, cabinet member for place shaping, said: “Crossness is a heritage site unlike any other in Bexley and a real hidden gem.
“It’s a truly awe-inspiring feat of engineering that many local people have yet to see.
“Crossness is celebrating its 160th birthday in the same year as the borough is celebrating its 60th anniversary and I am very pleased this special weekend of steaming and celebrations is featured in the #CelebratingBexley programme.”
Emma Jepson, trustee of Crossness Engines Trust, added: “We are thrilled to celebrate the 160th anniversary of Crossness, a testament to the dedication and hard work of our incredible volunteers.
“Their passion has preserved this magnificent example of Victorian engineering, ensuring its beauty and inspiration endure for future generations.
“Crossness is proud to be part of the #CelebratingBexley initiative, showcasing the borough’s most treasured venues.”
Tickets for the event and other event days throughout the year can be purchased at the Crossness Engines website.
For more information, visit the Bexley Council website.