Adam Glanville, 7, went missing in Richmond and police have not been able to track his whereabouts since June 3, 2024.
The boy is believed to be with his mum, Karima Mahmoud, 43, who is in breach of a family court order.
On October 24, officers received a sighting of Karima in the vicinity of Bedfont Road in Feltham.
Today (January 10) detectives have released images of two men they want to identify and speak to as part of the investigation.
While police do not believe these men have caused any harm to Adam or Karima, they think they can help trace them.
Detective Sergeant Mary Dawson, who is leading the investigation, said: “We need the public’s help to locate Karima and Adam.
“Karima is in breach of a family court order and police are trying to locate them both.
“I also want to identify and speak with the two men shown in the image released today.
“They were involved in an altercation with a man at the time Karima and Adam were last seen together on 3 June – the images were taken then.
“I do not believe they have caused any harm to Karima or Adam, but I believe they may have information as to their whereabouts.”
Anyone with information that may assist the investigation, including about the identity of the two men, is asked to call 101 quoting 01/903997/24.
To remain 100% anonymous contact the independent charity Crimestoppers on 0800 555 111.
For an immediate sighting, please dial 999.