Alexander-Arnold, who joined Real Madrid from Liverpool during the summer, has been restricted to just five appearances across all competitions for his new club so far this season.
However, as confirmed by Los Blancos, Alexander-Arnold – who completed a full training session on Friday as preparations began for the visit of Barcelona – is in the squad.
Trent Alexander-Arnold is in line to return to action against Barcelona
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In addition, fellow summer signing Dean Huijsen returns to the squad after missing wins over Getafe and Juventus with a calf injury sustained while on international duty with Spain earlier in the month.
Huijsen could partner Eder Militao in central defence as Antonio Rudiger and David Alaba will not feature at the weekend.
Meanwhile, rounding off the positive news for Madrid head coach Xabi Alonso, Dani Ceballos and club captain Dani Carvajal are available for selection against the reigning La Liga champions.
Confirmed Real Madrid squad
Goalkeepers: Courtois, Lunin, Sergio Mestre.
Defenders: Carvajal, Militao, Trent, Asencio, A. Carreras, Fran Garca, F. Mendy, Huijsen.
Midfielders: Bellingham, Camavinga, Valverde, Tchouameni, Arda Guler, D. Ceballos.
Forwards: Vini Jr., Endrick, Mbappe, Rodrygo, Gonzalo, Brahim, Mastantuono.
